Glossary entry (derived from question below)
French term or phrase:
1 dilution au 1/2
English translation:
a two-fold dilution
Added to glossary by
Drmanu49
Jan 1, 2009 15:06
15 yrs ago
French term
1 dilution au 1/2
French to English
Medical
Medical: Pharmaceuticals
bacterial endotoxins test
Hello
Happy New Year to you all!
If anyone can help with this query, I would be very grateful.
This comes under the heading "Gel formation/Gel clot technique":
Cet essai doit permettre de trouver une dilution du produit au moins inférieure ou égale a la DMS, non inhibitrice et non activatrice du test.
......
Pour la poursuite de la recherche des facteurs d'interférence, se reporter au chapitre EP XXXXii. Cette recherche est généralement confirmée sur 3 lots.
Résultat escompté: sensibilité déclarée du réactif a [with a grave accent] plus/minus 1 dilution au 1/2.
Thank you for any help.
Liz Askew
Happy New Year to you all!
If anyone can help with this query, I would be very grateful.
This comes under the heading "Gel formation/Gel clot technique":
Cet essai doit permettre de trouver une dilution du produit au moins inférieure ou égale a la DMS, non inhibitrice et non activatrice du test.
......
Pour la poursuite de la recherche des facteurs d'interférence, se reporter au chapitre EP XXXXii. Cette recherche est généralement confirmée sur 3 lots.
Résultat escompté: sensibilité déclarée du réactif a [with a grave accent] plus/minus 1 dilution au 1/2.
Thank you for any help.
Liz Askew
Proposed translations
(English)
| 4 +4 | dilution at 50% |
Drmanu49
|
| 5 | half concentration |
:::::::::: (X)
|
| 3 | +/- 1 ilution per 1/2 |
Sara Rodriguez
|
Proposed translations
+4
3 mins
Selected
dilution at 50%
and as the injected amount of serum was contained in 0.5 ml. the value in provisional units per milliliter equals. Serum dilution at 50 per cent endpoint X ...
www.jimmunol.org/cgi/reprint/38/5/399.pdf -
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Note added at 4 mins (2009-01-01 15:11:00 GMT)
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or at 0.5 would be the same.
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Note added at 21 hrs (2009-01-02 12:32:33 GMT) Post-grading
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#
nalytical Biochemistry : A rapid and sensitive method for the ...
- [ Traduire cette page ]
Analytical Biochemistry · Volume 72, Issues 1-2, 7 May 1976, Pages 248-254 .... Perhaps this results from increased dilution of the protein reagent. ...
linkinghub.elsevier.com/retrieve/pii/0003269776905273 - Pages similaires
de MM Bradford - 1976 - Cité 72230 fois - Autres articles - Les 15 versions
# [PDF]
Limulus Amebocyte Lysate
- [ Traduire cette page ]
Format de fichier: PDF/Adobe Acrobat - Version HTML
labeled sensitivity of the Pyrotell is confirmed if the endpoint is. l. plus or minus a twofold dilution. In this. example, the concentration of endotoxin ...
www.acciusa.com/pdfs/pisheets/Pyrotell STV Insert English -... -
www.jimmunol.org/cgi/reprint/38/5/399.pdf -
--------------------------------------------------
Note added at 4 mins (2009-01-01 15:11:00 GMT)
--------------------------------------------------
or at 0.5 would be the same.
--------------------------------------------------
Note added at 21 hrs (2009-01-02 12:32:33 GMT) Post-grading
--------------------------------------------------
#
nalytical Biochemistry : A rapid and sensitive method for the ...
- [ Traduire cette page ]
Analytical Biochemistry · Volume 72, Issues 1-2, 7 May 1976, Pages 248-254 .... Perhaps this results from increased dilution of the protein reagent. ...
linkinghub.elsevier.com/retrieve/pii/0003269776905273 - Pages similaires
de MM Bradford - 1976 - Cité 72230 fois - Autres articles - Les 15 versions
# [PDF]
Limulus Amebocyte Lysate
- [ Traduire cette page ]
Format de fichier: PDF/Adobe Acrobat - Version HTML
labeled sensitivity of the Pyrotell is confirmed if the endpoint is. l. plus or minus a twofold dilution. In this. example, the concentration of endotoxin ...
www.acciusa.com/pdfs/pisheets/Pyrotell STV Insert English -... -
Peer comment(s):
| agree |
Alazne Andia
48 mins
|
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Thank you Alazne.
|
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| agree |
Lionel_M (X)
: that's the more idiomatic way ! (sorry...each time I make a change I introduce another error....I for sure have a bug !)
1 hr
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Thank you Lionel.
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| agree |
Transitwrite
1 hr
|
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Thank you Sharon.
|
|
| agree |
Patricia Fierro, M. Sc.
1 hr
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Thank you Patricia.
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| disagree |
SJLD
: "dilution at 50%" does not mean dilute to a concentration of 50% - your ref is irrelevant/I know exactly what the French means thanks - but "dilution at 50%" is NOT the correct translation - find me a relevant ref and I might be convinced
7 hrs
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OK for a two-fold dilution.
|
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| agree |
Caterina Lanfranco
1 day 1 hr
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Thank you Caterina.
|
4 KudoZ points awarded for this answer.
Comment: "Thank you very much!"
5 mins
Sara Rodriguez
United States
United States
Native in English
Works in field
United States
Ready to work in English/Spanish/French
Native in: English (Variants: US, US South) 
, Spanish (Variant: US)
Works in: English to Spanish, Spanish to English, French to English, and 3 more.
+/- 1 ilution per 1/2
Plus or minus one dilution per 1/2 - as in 1 cup of water for every 1/2 cup of salt
9 mins
half concentration
1 Dilution an Oxymoron? - General Lab Techniques - [ Traduire cette page ]1:2 means half concentration. 1:3 means 1/3 concentration 1:10 mean 1/10 concentration. And why should 1:1 dilution be an oxymoron? ...
www.protocol-online.org/biology-forums/posts/31255.html - 16k - En cache - Pages similaires
SUE - SPERM DILUTION - [ Traduire cette page ]The result is now half the concentration of the original. If we now do a further 1:1 dilution of the dilution we have 1/2 times 1/2 or 1/4 the original ...
www.stanford.edu/group/Urchin/sperm-d.htm - 25k - En cache - Pages similaires
On the effect of a regular = 1 dilution of = 1 2 antiferromagnetic ... - [ Traduire cette page ]1/2 spins in a cell, i.e., S. eff. is half-integer, .... The magnetization versus the dilution concentration ρ = 1/K for β = 200 and L ∼ 200. ...
www.iop.org/EJ/article/0953-8984/17/36/010/cm5_36_010.pdf - Pages similaires
de F Jin - 2005 - Autres articles - Les 6 versions
[DOC] Determination of the pH Scale by the Method of Successive Dilutions - [ Traduire cette page ]Format de fichier: Microsoft Word - Version HTML
Now add the same amount of stock 1M HCl to the first flask (20 mL), and note that the concentration has been diluted in half, [
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Note added at 10 mins (2009-01-01 15:17:14 GMT)
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and NOT dilution at 50% which is not the same ratio
www.protocol-online.org/biology-forums/posts/31255.html - 16k - En cache - Pages similaires
SUE - SPERM DILUTION - [ Traduire cette page ]The result is now half the concentration of the original. If we now do a further 1:1 dilution of the dilution we have 1/2 times 1/2 or 1/4 the original ...
www.stanford.edu/group/Urchin/sperm-d.htm - 25k - En cache - Pages similaires
On the effect of a regular = 1 dilution of = 1 2 antiferromagnetic ... - [ Traduire cette page ]1/2 spins in a cell, i.e., S. eff. is half-integer, .... The magnetization versus the dilution concentration ρ = 1/K for β = 200 and L ∼ 200. ...
www.iop.org/EJ/article/0953-8984/17/36/010/cm5_36_010.pdf - Pages similaires
de F Jin - 2005 - Autres articles - Les 6 versions
[DOC] Determination of the pH Scale by the Method of Successive Dilutions - [ Traduire cette page ]Format de fichier: Microsoft Word - Version HTML
Now add the same amount of stock 1M HCl to the first flask (20 mL), and note that the concentration has been diluted in half, [
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Note added at 10 mins (2009-01-01 15:17:14 GMT)
--------------------------------------------------
and NOT dilution at 50% which is not the same ratio
Reference comments
1 hr
Reference:
Dilution
Peer comments on this reference comment:
| agree |
SJLD
: I would prefer two-fold dilution as in your ref - here too: http://www.fao.org/docrep/005/ac802e/ac802e0q.htm
42 mins
|
|
Thank you. Two-fold diln is fine too
|
7 hrs
Reference:
careful!
Travaux pratiques : ETUDE PRATIQUE DE QUELQUES RECEPTEURSDiluer 1 mL de la solution sucrée avec 1 mL d'eau distillée (dilution au 1/2). Tester si le sujet ressent une sensation sucrée sur la pointe de la langue. ...
www.didier-pol.net/2RECEP2.htm
As Lionel pointed out, this is known as a 1:1 dilution (equal volumes of solute and solvent) or 1/2 dilution (vol of solute/total vol final solution). The resulting solution has a concentration that is 50% of the initial solution.
"dilution at 50%" is not the correct term. People without knowledge of the exact terminology would do best not to mislead others. These terms cannot be arrived at by guessing and googling.
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Note added at 7 hrs (2009-01-01 22:57:15 GMT) Post-grading
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Re "dilution at 50% endpoint"
http://ecfr.gpoaccess.gov/cgi/t/text/text-idx?c=ecfr&sid=95d...
6) The relative potency (RP) of the Unknown is determined by comparing the 50 percent endpoint dilution (highest bacterin dilution protecting 50 percent of the mice) of the Unknown with that of the Standard by the following formula...
In the ref given by Mrmanu (which cannot be accessed by the way, which suggests that he hasn't actually read it), "Serum dilution at 50 per cent endpoint" means the dilution (concentration) at the point when 50% of the mice are still alive (i.e. 50% are dead). It has nothing to do with a 1/2 dilution.
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Note added at 7 hrs (2009-01-01 23:02:33 GMT) Post-grading
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http://www.sci.sdsu.edu/classes/chemistry/chem467l/mardahl/s...
http://faculty.sdmiramar.edu/dtrubovitz/micro/dilutions/dilu...
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Note added at 22 hrs (2009-01-02 13:40:14 GMT) Post-grading
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The plus or minus one two-fold dilution is the error of the sensitivity measurement. See the end of this package insert under "Limitations of the procedure".
http://www.criver.com/SiteCollectionDocuments/qc_mm_r_kta_pa...
--------------------------------------------------
Note added at 22 hrs (2009-01-02 13:47:39 GMT) Post-grading
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[PDF] GUIDELINE ON VALIDATION OF THE LIMULUS AMEBOCYTE LYSATE TEST AS AN ... - [ Traduire cette page ]Format de fichier: PDF/Adobe Acrobat - Version HTML
perform the test for confirmation of labeled LAL reagent sensitivity or of performance ... plus/minus a twofold dilution of the labeled sensitivity. ...
www.bcg-usa.com/regulatory/docs/1987/FDA198712A.pdf -
Endotoxins: Pyrogens, LAL Testing and Depyrogenation - Résultats Google Recherche de Livresde Kevin L. Williams - 2007 - Medical - 419 pages
Results of endotoxin determination in water and the drug product should fall within plus/minus a two-fold dilution of the labeled sensitivity. ...
books.google.fr/books?isbn=0849393728...
www.didier-pol.net/2RECEP2.htm
As Lionel pointed out, this is known as a 1:1 dilution (equal volumes of solute and solvent) or 1/2 dilution (vol of solute/total vol final solution). The resulting solution has a concentration that is 50% of the initial solution.
"dilution at 50%" is not the correct term. People without knowledge of the exact terminology would do best not to mislead others. These terms cannot be arrived at by guessing and googling.
--------------------------------------------------
Note added at 7 hrs (2009-01-01 22:57:15 GMT) Post-grading
--------------------------------------------------
Re "dilution at 50% endpoint"
http://ecfr.gpoaccess.gov/cgi/t/text/text-idx?c=ecfr&sid=95d...
6) The relative potency (RP) of the Unknown is determined by comparing the 50 percent endpoint dilution (highest bacterin dilution protecting 50 percent of the mice) of the Unknown with that of the Standard by the following formula...
In the ref given by Mrmanu (which cannot be accessed by the way, which suggests that he hasn't actually read it), "Serum dilution at 50 per cent endpoint" means the dilution (concentration) at the point when 50% of the mice are still alive (i.e. 50% are dead). It has nothing to do with a 1/2 dilution.
--------------------------------------------------
Note added at 7 hrs (2009-01-01 23:02:33 GMT) Post-grading
--------------------------------------------------
http://www.sci.sdsu.edu/classes/chemistry/chem467l/mardahl/s...
http://faculty.sdmiramar.edu/dtrubovitz/micro/dilutions/dilu...
--------------------------------------------------
Note added at 22 hrs (2009-01-02 13:40:14 GMT) Post-grading
--------------------------------------------------
The plus or minus one two-fold dilution is the error of the sensitivity measurement. See the end of this package insert under "Limitations of the procedure".
http://www.criver.com/SiteCollectionDocuments/qc_mm_r_kta_pa...
--------------------------------------------------
Note added at 22 hrs (2009-01-02 13:47:39 GMT) Post-grading
--------------------------------------------------
[PDF] GUIDELINE ON VALIDATION OF THE LIMULUS AMEBOCYTE LYSATE TEST AS AN ... - [ Traduire cette page ]Format de fichier: PDF/Adobe Acrobat - Version HTML
perform the test for confirmation of labeled LAL reagent sensitivity or of performance ... plus/minus a twofold dilution of the labeled sensitivity. ...
www.bcg-usa.com/regulatory/docs/1987/FDA198712A.pdf -
Endotoxins: Pyrogens, LAL Testing and Depyrogenation - Résultats Google Recherche de Livresde Kevin L. Williams - 2007 - Medical - 419 pages
Results of endotoxin determination in water and the drug product should fall within plus/minus a two-fold dilution of the labeled sensitivity. ...
books.google.fr/books?isbn=0849393728...
Discussion
The French text does not have 1/2 in large figures but as small ones, i.e. expressed as a half.
I did not make this at all clear in my query.
sorry to be thick, but is this still "two-fold dilution"?
Many, many thanks for your intelligent intervention. It would have been a poor mistake in my translation indeed :-) The awful thing is that I am no scientist, so I am hoping to God that there is an excellent proofreader out there who will be reading my end translation. What more can one do, eh?
I may have just twigged, I think this =
1:0.5 dilution